Biology Blog #3a: PCR vs. Vector Cloning - October 8, 2011

Polymerase Chain Reaction:

1. it's a technique used to replicate the same small strand of DNA over and over again. 
2. DNA is denatured at high temperatures
3. five steps to it: 
1. Initialization step:
2. Denaturation step: 
3. Annealing step: 
4. Extension/elongation step: 
5. Final elongation: 
6. Final hold

Vector Cloning:

1. restriction enzymes used to cut gene of interest and original at specific points so that the sticky ends match
2. DNA must be artificially created to ensure that the gene is there.